Hong Jiang
Qingdao University, China
Title: Nesfatin-1 protects dopaminergic neurons against MPTP neurotoxicity through C-Raf/ERK1/2 dependent anti-apo
Biography
Biography: Hong Jiang
Abstract
Some brain-gut peptides have been reported to have a close relationship with the central dopamine system. Nesfatin-1, a satiety brain-gut peptides co-expressed with ghrelin in X/A like endocrine cells in the gastric glands, has been reported exerted neuroprotective efficacy in center neurons system. Here we defined the neuroprotective effects of nesfatin-1 against MPTP-induced dopaminergic neuron degeneration in vivo and MPP+-induced cytotoxicity in vitro with its anti-apoptotic action via ameliorating mitochondrial dysfunction by activation of C-Raf/ERK1/2 pathway. In MES23.5 dopamingernic cells, nesfatin-1 pretreatment antagonized MPP+-induced mitochondrial dysfunction related apoptosis cascades including ΔΨm collapse, mitochondrion Cyt C releasing, caspase-3 activation and morphological changes of nuclei. This protective effect was abolished by selective inhibitor of C-Raf and ERK1/2. In C56BL/6 mouse, intracerebroventricular nesfatin-1 pretreatment attenuated MPTP-induced dopaminergic neuronal degeneration in the SNpc, dopaminergic fibers depletion, dopamine and its metabolites contents depletion in the striatum. Our data suggested nesfatin-1 had the potential to be considered as an agent for therapy of Parkinson’s disease.